br Conclusion br In conclusion we developed a target
In conclusion, we developed a target drug delivery system to en-hance cancer targeting under a static magnetic field and laser irradia-tion to inhibit tumor growth. The system was demonstrated to be ef-fective for the synchronization of in situ transdermal administration, a magnetic field, and laser and biological targeting. To inducing DNA damage signaling and increasing the concentration of free radicals in breast tumor cells, which are triggered by PDT with HMME and DOX chemotherapy. These results suggest that laser-activatable and mag-netic-targeting LMNs exert a synergistic eﬀect on breast cancer inhibi-tion and it is an eﬀective, and alternative option for superficial cancer treatment. Most importantly, the synthesized LMNs absorbed into the BC may oﬀer a solution for challenges in cancer treatment, namely, limited penetration depth and oxygen-deficient microenvironments.
The expenses of this work were supported by the National Natural Science Foundation of China (31370967, 31170919), China, the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2014), China, the Science and Technology Planning Project of Guangdong Province (2015A020212033), China, the Science
Appendix A. Supplementary data
K. Harper, E. Tardio, I. Reyes Torres, J. Jones, J. Condeelis, M. Merad, J.A. Aguirre-Ghiso, Macrophages orchestrate breast cancer early dissemination and metastasis, Nat. Commun. 9 (2018) 1–14.  S. Safwat, R.M. Hathout, R.A. Ishak, N.D. Mortada, Augmented simvastatin cyto-toxicity using optimized lipid nanocapsules: a potential for breast cancer treatment, J. Liposome Res. 27 (2017) 1–10.
 S.W. Du, L.K. Zhang, K. Han, S. Chen, Z. Hu, W. Chen, K. Hu, L. Yin, B. Wu, Y.Q. Guan, Combined phycocyanin and hematoporphyrin monomethyl ether for breast cancer treatment via photosensitizers modified Fe3O4 nanoparticles in-hibiting the proliferation and AS1517499 of MCF-7 cells, Biomacromolecules 19 (2018) 31–41.
 J. Nam, S. Son, L.J. Ochyl, R. Kuai, A. Schwendeman, J.J. Moon, Chemo-photo-thermal therapy combination elicits anti-tumor immunity against advanced meta-static cancer, Nat. Commun. 9 (2018) 1074.  S.M. Abozeid, R.M. Hathout, K. Abou-aisha, Silencing of the metastasis-linked gene, AEG-1, using siRNA-loaded cholamine surface-modified gelatin nanoparticles in the breast carcinoma cell line MCF-7, Colloids Surf. B Biointerfaces 145 (2016)
J. Wang, Macrophage-Specific in vivo gene editing using cationic lipid-assisted polymeric nanoparticles, ACS Nano 12 (2018) 994–1005.
 S.M. Abdel-Hafez, R.M. Hathout, O.A. Sammour, Curcumin-loaded ultradeformable nanovesicles as a potential delivery system for breast cancer therapy, Colloids Surf. B Biointerfaces 167 (2018) 63–72.  R. Yehia, R.M. Hathout, D.A. Attia, M.M. Elmazar, N.D. Mortada, Anti-tumor eﬃ-cacy of an integrated methyl dihydrojasmonate transdermal microemulsion system targeting breast cancer cells: In vitro and in vivo studies, Colloids Surf. B Biointerfaces 155 (2017) 512–521.  S.M. Abdel-Hafez, R.M. Hathout, O.A. Sammour, Tracking the transdermal pene-tration pathways of optimized curcumin-loaded chitosan nanoparticles via confocal laser scanning microscopy, Int. J. Biol. Macromol. 108 (2018) 753–764.
G. Cremaschi, E. Rivera, V. Medina, Selective cytoprotective eﬀect of histamine on doxorubicin-induced hepatic and cardiac toxicity in animal models, Cell Death Discov. 1 (2015) 15059.
S. Huang, X. Pan, K. Li, R. Schiﬀ, X. Wang, Comprehensive functional analysis of the tousled-like kinase 2 frequently amplified in aggressive luminal breast cancers, Nat. Commun. 7 (2016) 1–17.
C. Jiang, M. Akram, E. Brogi, B. Leitinger, F. Giancotti, Multi-organ site metastatic reactivation mediated by non-canonical discoidin domain receptor 1 signaling, Cell 166 (2016) 47–62.
O. Mykhaylyk, D. Eberbeck, D. Wenzel, A. Pfeifer, Targeting of magnetic nano-particle-coated micro- bubbles to the vascular wall empowers site-specific lentiviral gene delivery in vivo, Theranostics 7 (2017) 295–307.
D. Letourneur, C. Wilhelm, Combining magnetic nanoparticles with cell derived Chemical Engineering Journal 370 (2019) 749–759
 X. Zhan, Y. Guan, Design of magnetic nanoparticles for hepatocellular carcinoma treatment using the control mechanisms of cell internal nucleus and external membrane, J. Mater. Chem. B. (2015) 1–7.  C.J. Cheng, G.T. Tietjen, J.K. Saucier-Sawyer, W.M. Saltzman, A holistic approach to targeting disease with polymeric nanoparticles, Nat. Rev. Drug Discov. 14 (2015) 239–247.
 N. Kotagiri, G.P. Sudlow, W.J. Akers, S. Achilefu, Breaking the depth dependency of phototherapy with Cerenkov radiation and low-radiance-responsive nanophoto-sensitizers, Nat. Nanotechnol. 10 (2015) 370–379.